Laser scanning confocal microscope Leica SP8 X FLIM

Laser scanning confocal microscope Leica SP8 X FLIM

  • Skraćeni naziv instrumenta

    New confocal

    Inventarni broj

    0042863

    Opći opis

    Confocal laser scanning microscope is a light microscope used for monitoring and recording of living and inanimate microscopic specimens using fluorescence and reflected light. In this method, the specimen is illuminated point by point, and reemitted or reflected light is detected and recorded to build an image in computer memory. The main advantage of confocal microscopy vs. wide-field microscopy is its capability for optical sectioning, i.e. obtaining a sharp image of a thin section of a thick specimen. Confocal microscopy has a wide scope of applications similar to classical light microscopy. Our instrument is based on an inverse microscope.

    Uža područja primjene

    Light imaging of mainly biological and biomedical samples

    Namjena instrumenta

    Imaging of microstructures

    Popis usluga

    Confocal imaging; fluorescence lifetime imaging microscopy (FLIM)

    Project title

    Enhancement of the innovation potential in SEE through new molecular solutions in research and development–InnoMol, FP7-RegPot

    Ključne riječi

    Fluorescence, optical sectioning, FLIM

    Procijenjeni broj korisnika

    40

    Year of manufacture

    2013

  • Ustanova en

    Institut Ruđer Bošković
    Bijenička cesta 54, Zagreb

    Zavod

    Division of molecular biology

    Laboratorij

    Laboratory of cell biophysics

    Grad

    Zagreb

    Ulica i broj

    Bijenička 54

    Krilo/Kat/Soba

    5. wing / basement / 014B

  • Način korištenja instrumenta

    , ,

    Broj istovrsnih instrumenata u ustanovi

    2

  • Model

    Leica TCS SP8 X FLIM

    Mrežna (URL) adresa instrumenta na stranicama proizvođača

    http://www.leica-microsystems.com/products/confocal-microscopes/details/product/leica-tcs-sp8-x/

    Princip rada i mjerna tehnika

    confocal fluorescence microscopy

    Radno i mjerno područje

    Fluorescence and reflection microscopy, FLIM

    Proizvođač

    Leica Microsystems

    Mrežna (URL) adresa proizvođača

    http://www.leica-microsystems.com/

    Purchase price

    5174800

    Vijek trajanja

    10 years

    Ostale tehničke karakteristike

    Confocal laser scanning microscope is a light microscope used for monitoring and recording of living and inanimate microscopic specimens using fluorescence and reflected light. In this method, the specimen is illuminated point by point, and reemitted or reflected light is detected and recorded to build an image in computer memory. The main advantage of confocal microscopy vs. wide-field microscopy is its capability for optical sectioning, i.e. obtaining a sharp image of a thin section of a thick specimen. Confocal microscopy has a wide scope of applications similar to classical light microscopy. Our instrument is based on an inverse microscope.

    Detaljne tehničke karakteristike

    Five discrete excitation laser lines: 405, 458, 488, 496 and 514 nm, and the supercontinuum excitation range from 470 to 700 nm. Laser intensity of 8 excitation lines can be adjusted continuously and independently. Four detection channels. Spectral detection system, AOBS, classic and resonant scanner, x, y, z, t and double lambda imaging modes. 12 bit dynamics, FRAP, FRET, FLIM.

    Popratna i dodatna oprema

    A system for maintenance of controlled conditions, temperature, atmosphere and humidity, in the specimen chamber. Programme for image visualization and analysis Huygens Professional.

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