Modular high performance liquid chromatography (HPLC), PerkinElmer HPLC Series 200, with DAD detector Series 200 EP (UV/VIS range 190-700 nm), autosampler Series 225 with Peltier tray, quaternary pump, 5-channel vacuum degassing system, Peltier column oven, Gilson fraction collector and software packages TotalChrom Workstation and IRIS TurboScan200. Instrumental set-up equipped with size-exclusion column is used for the efficient and reproducible separation of cytosolic biomolecules isolated from the tissues of indigenous fish and shellfish specimens, collected at marine and freshwater sampling sites differently affected by metal pollution.
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