Pulsed-field gel electrophoresis (PFGE)

Pulsed-field gel electrophoresis (PFGE)

  • Skraćeni naziv instrumenta

    PFGE

    Inventarni broj

    S08270

    Opći opis

    An alternating electric field between spatially different pairs of electrodes causes megabase (Mb) DNA to reorient and move at different speeds through pores in an agarose gel. The voltage changes periodically between three directions; one passing through the central axis of the gel and two extending at a 60-degree angle on either side. Pulse times are the same for each direction resulting in net DNA migration forward. For extremely large molecules, switching interval ramps can be used that increase the pulse time for each direction over a specified number of hours. The CHEF Mapper system separates large and small DNA fragments with better resolution, speed and accuracy than traditional field pulsation methods. The method effectively separates DNA sizes ranging from 100 pb to over 10 Mb.

    Uža područja primjene

    Microbiology, molecular biology, genetics, tumor research, public health

    Namjena instrumenta

    Pulsed field gel electrophoresis system (PFGE) is a technique for separating DNA molecules up to approx. 10 Mb by their reorientation and movement at different speeds through the pores of the agarose gel. PFGE is used for genotyping or determining the gen

    Project title

    Functional integration of the University of Split, PMF-ST, PF-ST and KTF-ST through the development of scientific research infrastructure in the building of three faculties (KK.01.1.1.02.0018)

    Type of the analysis

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    Applications

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    Ključne riječi

    DNA analysis, genotyping

    Procijenjeni broj korisnika

    5

    Year of manufacture

    2020

  • Ustanova en

    Sveučilište u Splitu, Prirodoslovno-matematički fakultet
    Ruđera Boškovića 33, Split

    Zavod

    Department of biology

    Laboratorij

    Laboratory for microbiology

    Grad

    Split

    Ulica i broj

    Ruđera Boškovića 33

    Krilo/Kat/Soba

    2nd floor/B2-64

  • Način korištenja instrumenta

    ,

    Servis uz naplatu

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  • Ime i prezime

    Ana Maravić

    Titula

    Associate Professor, PhD

    E-mail

    ana.maravic@pmfst.hr

    Telefon

    +385 21 619 281

  • Model

    CHEF Mapper XA Chiller System

    Mrežna (URL) adresa instrumenta na stranicama proizvođača

    https://www.bio-rad.com/en-hr/product/chef-mapper-xa-system?ID=9153c7d0-407e-433e-8411-056120984257

    Princip rada i mjerna tehnika

    An alternating electric field between spatially different pairs of electrodes causes megabase (Mb) DNA to reorient and move at different speeds through pores in an agarose gel. The voltage changes periodically between three directions; one passing through the central axis of the gel and two extending at a 60-degree angle on either side. Pulse times are the same for each direction resulting in net DNA migration forward. For extremely large molecules, switching interval ramps can be used that increase the pulse time for each direction over a specified number of hours. The CHEF Mapper system separates large and small DNA fragments with better resolution, speed and accuracy than traditional field pulsation methods. The method effectively separates DNA sizes ranging from 100 pb to over 10 Mb.

    Proizvođač

    Bio-Rad, USA

    Mrežna (URL) adresa proizvođača

    https://www.bio-rad.com/

    Purchase price

    527.333

    Ostale tehničke karakteristike

    • The system uses CHEF, PACE, DR, FIGE and AFIGE technology.
    • Possibility of separating fragments from min. 100bp to 10Mb.
    • Built-in power supply of 350 V max. , gradient of 9 V / cm and continuous adjustment.
    • Battery support of working memory in order to enable memory of all set parameters and progress of protocol execution in case of power failure
    • Possibility of delayed start of at least 72 hours.
    • Electrode potential of the system dynamically regulated +/- 0.5% F.S.
    • Switching range of min. 50 ms to> 10 hours.
    • Variable switching angle from 0 to 360 ° completely electronic with a change in steps of 0.5 °.
    • Up to 15 switching vectors per cycle defined, by angle, voltage and duration.
    • Secondary pulse determined by voltage, frequency, angle and time.
    • Ability to work with 8 program blocks with automatic execution.
    • Maximum driving time of 999 hours per block.
    • The electrophoresis unit of 24 platinum electrodes.

    Mrežna (URL) adresa s tehničkim karakteristikama

    https://www.bio-rad.com/en-hr/product/chef-mapper-xa-system?ID=9153c7d0-407e-433e-8411-056120984257

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