Time resolved fluorescence (TRF) fluorimeter Edinburgh Inst. FS5

Time resolved fluorescence (TRF) fluorimeter Edinburgh Inst. FS5

  • Skraćeni naziv instrumenta

    TRF fluorimeter

    Inventarni broj

    47958

    Opći opis

    The instrument for stationary and time-resolved fluorescence (TRF) can record the fluorescence spectra of common solutions and solid samples, but the special feature of this instrument is the possibility of time-resolved fluorescence measurements of time-resolved fluorescence solutions with a resolution below 1 ns, which allows accurate and precise characterization of any fluorescent solution.

    Uža područja primjene

    Study of fluorescent materials and solutions

    Namjena instrumenta

    Study of fluorescent materials and solutions

    Popis usluga

    Study of fluorescent materials and solutions

    Project title

    Horizon 2020 FETPROACT-EIC-06-2019

    Type of the analysis

    ,

    Applications

    , , , , , , , , , , , , ,

    Ključne riječi

    fluorescence; time-resolved fluorescence

    Procijenjeni broj korisnika

    >100

    Year of manufacture

    2020

  • Ustanova en

    Institut Ruđer Bošković
    Bijenička cesta 54, Zagreb

    Zavod

    Zavod za organsku kemiju i biokemiju

    Laboratorij

    Laboratory for Biomolecular Interactions and Spectroscopy

    Grad

    Zagreb

    Ulica i broj

    Bijenička c. 54

    Krilo/Kat/Soba

    4. krilo, 1 kat, soba 4c

  • Način korištenja instrumenta

    ,

    Cjenik korištenja

    The instrument is 100% engaged with research within the Horizon 2020 project FETPROACT-EIC-06-2019 from which funds it was procured and will be available to other users only from 2022, when the project is completed.

    Popis ovlaštenih korisnika

    Ivo Piantanida
    Nikola Basarić

  • Ime i prezime

    Ivo Piantanida

    Titula

    Dr. sc.

    E-mail

    pianta@irb.hr

    Telefon

    1466

  • Model

    Edinburgh Inst. FS5

    Mrežna (URL) adresa instrumenta na stranicama proizvođača

    https://www.edinst.com/us/products/fs5-spectrofluorometer/

    Princip rada i mjerna tehnika

    Fluorescence spectrophotometry, pulse lasers of various wavelengths with a resolution of <1ns.

    Radno i mjerno područje

    fluorescence 200 nm-1000 nm

    Proizvođač

    Edinburgh Inst.

    Mrežna (URL) adresa proizvođača

    https://www.edinst.com/us/products/fs5-spectrofluorometer/

    Purchase price

    640659

    Vijek trajanja

    20 years

    Ostale tehničke karakteristike

    Light Source
    - 150 W xenon lamp

    Optical System
    - All reflective
    - Czerny Turner Monochromators (both excitation and emission) must have over 220mm focal length, and have grating turrets accepting up to 2 gratings.
    - Bandpass from 0* to 30 nm, continuously adjustable via software (*resolution limit of 0.3 nm)
    - Wavelength accuracy ±0.5 nm
    - Scan speed 100 nm/s
    - Sensitivity > 6000:1 (SQRT Method)

    Excitation Monochromator
    - Mechanical wavelength coverage: 200 nm-1000 nm
    - 1200 g/mm grating optimised for 300 nm

    Filter wheels
    Fully automatic filter wheels both in excitation and emission path to remove unwanted second and third-order grating effects.

    Detectors
    Reference Detector
    - Calibrated silicon reference detector
    Transmission Detector
    - Detector for transmission/absorption measurements must be included to make absorption measurement

    Emission Monochromator
    - Mechanical wavelength coverage: 200 nm-1000 nm
    - 1200 g/mm grating optimised for 500 nm
    - Automatic filter wheel for second order removal

    Emission Detector
    - Time-correlated Single photon counting (TCSPC) photomultiplier in temperature stabilised housing
    - TCSPC capability, including an electronic card with at least 305 fs/channel bin width, <25 ps electronic jitter and reverse mode automation.
    - Spectral range: 250 nm -850 nm or broader range
    Data Acquisition Electronics
    - Three parallel counter modules for fluorescence, reference and transmission detector. This allows simultaneous correction of excitation intensity.
    Guaranteed SQRT or FSD Signal to Noise Ratio of at least 6,000:1 for the water Raman signal (excitation at 350 nm, emission at 397 nm, with a 1 second integration time and 5 nm spectral bandwidth). Background signal defined as amplitude at 450nm, without background subtraction and without averaging.

    Software: for fluorescence software
    Set of lasers/diodes 340, 405, 450, 560 nm

    Mrežna (URL) adresa s tehničkim karakteristikama

    https://www.edinst.com/us/products/fs5-spectrofluorometer/

    Popratna i dodatna oprema

    Termostates

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