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Nikon ECLIPSE TE300 inverted microscope with micromanipulator

• Skraćeni naziv instrumenta

  Nikon Eclipse TE300 with micromanipulation system

  Opći opis

  The Nikon Eclipse TE300 is a high-end research microscope. Inverted microscope, modular building with upgrade capability (fluorescence, cell chamber, phase contrast).
  The micromanipulation system Narishige is mounted on the microscope with a joystick micromanipulator MN-151, pneumatic microinjector IM-9C and microinjector IM-9B. The microscope is equipped with a Color CCD camera.
  

  Uža područja primjene

  Embryology, micromanipulation in vitro, genetic modifications

  Namjena instrumenta

  Micromanipulation, microinjection, histological vizualization

  Popis usluga

  Embryo microinjection

  Ključne riječi

  inverted microscope, micromanipulator

  Procijenjeni broj korisnika

  15

  Year of manufacture

  2001

• Ustanova en

  Sveučilište u Zagrebu, Medicinski fakultet
  Šalata 3, Zagreb

  Zavod

  Department of Medical Biology

  Laboratorij

  Laboratory for in vitro culture

• Način korištenja instrumenta

  , ,

• Opis tržišne prilike

  The Nikon Eclipse TE300 is the only microscope in Zagreb where micromanipulation and microinjection of embryos of laboratory animals (mouse, rat) can be performed. On the microscope, it is possible to use new genetic engineering technologies involving the introduction of designed vector systems (CRISPR/Cas, RNAi). As such, it is open to all laboratories / research groups interested in this technology in the Republic of Croatia and neighboring countries.

  Opis usluge

  
  
  
  
  The openness to the external research community is expressed in the organization of training for methodology of work with the embryos, or in the form of expertise in national and international collaborative projects.
  Expertise includes: visualization, inspection and recording of microscopic preparations and micromanipulation of embryos of pre-implantation and post-implantation stages and cell cultures, including direct transfection of genetic material by microinjection.
  

• Princip rada i mjerna tehnika

  Inverted modular microscope with light source and condenser above the stage and objectives under the stage. Visualisation of the microscope slide or cell culture under optimal level of brightness, contrast, resolution and sharpness. Micromanipulation of animal embryo and transformation of animal genetic material.

  Radno i mjerno područje

  Morphology and size of material into micrometers, 22 millimeter field of view, injection in picoliters

  Purchase price

  300000

  Datum nabave

  1.1.2002

  Estimated current value of equipment

  200000

  Vijek trajanja

  Unlimited

  Popratna i dodatna oprema

  Termoplate for cell culture observation
  Pneumatic Injector IM-9C
  Microinjector IM-9B
  Joystick Micromanipulator MN-151
  Color CCD Camera Model ICD-803P

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