ThermoFisher Scientific Attune flow cytometer

ThermoFisher Scientific Attune flow cytometer

  • Skraćeni naziv instrumenta

    Attune

    Inventarni broj

    28731

    Opći opis

    Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations and analyzing cell size. It allows simultaneous multi-parameter analysis of single cells.

    It is predominantly used to measure fluorescence intensity produced by fluorescent-labeled antibodies detecting proteins, or ligands that bind to specific cell-associated molecules such as propidium iodide binding to DNA.

    The staining procedure involves making a single-cell suspension from cell culture or tissue samples. The cells are then incubated in tubes with fluorochrome-labeled antibodies and analyzed on the flow cytometer.

    Uža područja primjene

    medicine, immunology, haematology

    Namjena instrumenta

    cellullar phenotypization and analysis of cell subpopulations

    Popis usluga

    development and optimization of a phenotypization and analysis protocol for particular cell population, cell sorting and phenotypization, collected data analysis and statistics

    Ključne riječi

    flow cytometry, phenotypization

    Procijenjeni broj korisnika

    20

    Year of manufacture

    2013

  • Ustanova en

    Sveučilište u Zagrebu, Medicinski fakultet
    Šalata 3, Zagreb

    Zavod

    Croatian Institute for Brain Research

    Laboratorij

    Laboratory for Molecular Immunology

    Grad

    Zagreb

    Ulica i broj

    Šalata 12

    Krilo/Kat/Soba

    1st floor, room 114

  • Način korištenja instrumenta

    , ,

    Popis ovlaštenih korisnika

    prof. dr. sc. Danka Grčević
    prof. dr. sc. Nataša Kovačić

    Broj istovrsnih instrumenata u ustanovi

    1

  • Opis usluge

    Preparation, tuning, optimization and implementation of cell phenotypization protocols and analysis of results in agreement with associates. Collaboration can be achieved through joint projects or as a service.

    Status intelektualnog vlasništva

    on a case by case basis, as agreed with the client/partner/collaborator

  • Ime i prezime

    Danka Grčević

    Titula

    prof.

    E-mail

    danka.grcevic@mef.hr

    Telefon

    0038514566944

  • Model

    ThermoFisher Original Attune flow cytometer

    Mrežna (URL) adresa instrumenta na stranicama proizvođača

    https://www.thermofisher.com/hr/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/resources-original-attune-cytometer.html

    Radno i mjerno područje

    fluorescence of fluorophore-bound antibodies on cellular markers

    Proizvođač

    ThermoFisher Scientific

    Mrežna (URL) adresa proizvođača

    www.thermofisher.com

    Purchase price

    650000

    Estimated current value of equipment

    600000

    Estimated value of procurement of new equipment with identical characteristics

    750000HRK

    Vijek trajanja

    8 years

    Ostale tehničke karakteristike

    8 parameters - 2 lasers (red+blue) with 6 detection channels + FSC/SSC

    Detaljne tehničke karakteristike

    8 parameters - 2 lasers (red+blue) with 6 detection channels + FSC/SSC

    Mrežna (URL) adresa s tehničkim karakteristikama

    https://www.thermofisher.com/hr/en/home/life-science/cell-analysis/flow-cytometry/flow-cytometers/resources-original-attune-cytometer.html

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