BD FACSAria II cell sorter
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Skraćeni naziv instrumenta
BD FACSAria II
Inventarni broj
23195
Opći opis
Fluorescent Activated Cell Sorting, or FACS, utilizes Flow cytometry to provide a fast, objective and quantitative measurement of intra- and extracellular properties, not including morphology, for sorting a heterogeneous mixture of cells. This is the most common method of currently separating cells and involves encapsulating cells into small liquid droplets and selectively labeled with electric charges and sorted by an external electric field. This instrument is fitted with three lasers with nine channels in total for detection and analysis of cell markers, and with a possibility of simultaneous sorting of four cell populations from a given sample.
Uža područja primjene
medicine, immunology, haematology
Namjena instrumenta
fluorescence activated cell sorting
Popis usluga
development and optimization of a sorting protocol for particular cell population, cell sorting and phenotypization, collected data analysis and statistics
Tijelo koje je financiralo nabavku opreme
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Project title
Ministry of Health funded the acquisition of the BD FACSAria I instrument in 2007 for Clinical Hospital Centre Zagreb, in 2017 the instrument was handed over to University of Zagreb Medical School and upgraded to BD FACSAria II by funding from Centre for
Ključne riječi
cell sorter, flow cytometry, phenotypization, sorting
Procijenjeni broj korisnika
20
Year of manufacture
2007
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Ustanova en
Sveučilište u Zagrebu, Medicinski fakultet
Šalata 3, ZagrebZavod
Croatian Institute for Brain Research
Laboratorij
Laboratory for Molecular Immunology
Grad
Zagreb
Ulica i broj
Šalata 12
Krilo/Kat/Soba
2nd floor
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Način korištenja instrumenta
, ,
Popis ovlaštenih korisnika
prof. dr. sc. Danka Grčević
prof. dr. sc. Nataša KovačićBroj istovrsnih instrumenata u ustanovi
1
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Opis tržišne prilike
The Faculty of Medicine of the University of Zagreb is the only institution in Zagreb that possesses such a functional device available for research and experimental needs. We are also the only institution in Zagreb that regularly uses it and, moreover, in various domestic and foreign research projects. Because of the experience and the knowledge of the method, we are able to offer and achieve successful cooperation with other scientific groups in the country and region - wher, after agreeing on the required population, we conduct the procedure, analysis and sorting completely independent within the Medical Faculty.
Opis usluge
Preparation, tuning, optimization and implementation of cell sorting protocols and analysis of results in agreement with associates. Collaboration can be achieved through joint projects or as a service.
Status intelektualnog vlasništva
on a case by case basis, as agreed with the client/partner/collaborator
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Ime i prezime
Danka Grčević
Titula
prof.
E-mail
danka.grcevic@mef.hr
Telefon
0038514566944
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Model
BD FACSAria II
Mrežna (URL) adresa instrumenta na stranicama proizvođača
http://www.bdbiosciences.com/us/instruments/research/cell-sorters/bd-facsaria-iii/m/744763/overview
Princip rada i mjerna tehnika
Fluorescent Activated Cell Sorting, or FACS, utilizes Flow cytometry to provide a fast, objective and quantitative measurement of intra- and extracellular properties, not including morphology, for sorting a heterogeneous mixture of cells. This is the most common method of currently separating cells and involves encapsulating cells into small liquid droplets and selectively labeled with electric charges and sorted by an external electric field. This instrument is fitted with three lasers with nine channels in total for detection and analysis of cell markers, and with a possibility of simultaneous sorting of four cell populations from a given sample.
Radno i mjerno područje
fluorescence of fluorophore-bound antibodies on cellular markers
Proizvođač
Becton Dickinson
Mrežna (URL) adresa proizvođača
http://www.bdbiosciences.com
Purchase price
3500000
Estimated current value of equipment
3000000
Estimated value of procurement of new equipment with identical characteristics
3500000kn
Vijek trajanja
20 years
Ostale tehničke karakteristike
11 parameters - 3 lasers with 9 detection channels + FSC/SSC; simultaneous 4-way sort
Detaljne tehničke karakteristike
11 parameters - 3 lasers with 9 detection channels + FSC/SSC; simultaneous 4-way sort
Mrežna (URL) adresa s tehničkim karakteristikama
https://www.bdbiosciences.com/documents/BD_FACSAria_II_User_Guide.pdf
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